expresního systému pro produkci fosfolipázy D u Bacillus subtilis
Neutronová spinová echo spektroskopie jako jedinečná sonda professional lipidovou membránovou dynamiku a interakce membrána- protein
Lipidové dvojvrstvy tvoří hlavní matrici buněčných membrán a jsou mimo jiné životně důležitými buněčnými procesy primární platformou professional výměnu živin, interakce protein-membrána a virové pučení. Professional účinnou biologickou aktivitu by buněčné membrány měly být dostatečně tuhé, aby udržovaly integritu buňky a jejích oddílů, ale dostatečně tekuté, aby umožňovaly difúzi a interakci membránových složek, jako jsou proteiny a funkční domény.
Tato křehká rovnováha vlastností elastické a tekuté membrány a jejich dopad na biologickou funkci vyžaduje lepší pochopení dynamiky kolektivní membrány přes mezoskopickou délku a časovou škálu klíčových biologických procesů, např. Deformace membrány a vazebné události professional proteiny. Mezi techniky, které mohou účinně zkoumat tento dynamický rozsah, patří spektroskopie neutronové spinové echo (NSE).
V kombinaci s deuteriovým značením lze NSE použít okay přímému přístupu okay fluktuacím ohybu a tloušťky, stejně jako okay mezoskopické dynamice vybraných vlastností membrány. Tento článek poskytuje krátký popis techniky NSE a nastiňuje postupy professional provádění experimentů NSE na liposomálních membránách, včetně podrobností o přípravě a deuteraci vzorků, spolu s pokyny professional sběr a redukci dat.
Příspěvek také představuje metody analýzy dat používané okay extrakci klíčových parametrů membrány , jako je modul tuhosti v ohybu, modul stlačitelnosti oblasti a viskozita v rovině. Professional ilustraci biologického významu studií NSE jsou diskutovány vybrané příklady membránových jevů sondovaných NSE, jmenovitě účinek přísad na tuhost v ohybu membrány, dopad tvorby domény na fluktuace membrány a dynamický podpis interakcí membrána-protein
Porozumění jednotlivým proteinům SARS-CoV-2 professional cílený vývoj léčiv proti COVID-19
SARS-CoV-2 způsobuje pandemii COVID-19 zodpovědnou za miliony úmrtí na celém světě. I při účinných vakcínách si virus SARS-CoV-2 pravděpodobně udrží populaci v lidské populaci díky nedostatkům v účinnosti a vakcinaci a vzniku nových kmenů . Porozumění tomu, jak SARS-Cov-2 způsobuje tak rozsáhlé poškození tkání, a vývoj cílené farmakologické léčby bude proto v boji proti tomuto viru a při přípravě na další ohniska kritické.
Zde shrnujeme dosavadní pokrok pomocí modelů in vitro nebo in vivo ke zkoumání jednotlivých proteinů SARS-CoV-2 a jejich patogenních mechanismů. Seskupili jsme proteiny SARS-CoV-2 do tří kategorií: vstup professional hostitele, samočinný a interagující s hostitelem. Tato recenze se zaměřuje na samočinné a na hostitele interagující proteiny SARS-CoV-2 a shrnuje současné znalosti o tom, jak tyto proteiny podporují replikaci virů a narušují hostitelské systémy, stejně jako léky, které se zaměřují na tyto viry a interagující hostitelské proteiny.
Mnoho z těchto léků je v současné době v klinických studiích professional léčbu COVID-19. Budoucí propuknutí koronaviru bude pravděpodobně způsobeno novými virovými kmeny, které se vyhýbají vakcínám prostřednictvím mutací proteinů vstupujících do hostitele. Studium jednotlivých samostatně působících a na hostitele interagujících proteinů SARS-CoV-2 professional cílené terapeutické intervence je proto nejen nezbytné professional boj s COVID-19, ale také cenné proti budoucím ohniskům koronaviru.
inolab
Ribonuclease III, Nuclear (RNASEN) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1). This antibody is labeled with APC-Cy7.
Nuclear Mitotic Apparatus Protein 1 (NUMA1) Monoclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Pig Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Human, Mouse Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Human Aryl Hydrocarbon Receptor Nuclear Translocator 2 (ARNT2). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Rat Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP). This antibody is labeled with APC-Cy7.
HAUS Augmin Like Complex Subunit 7 (HAUS7) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Rat Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Human, Rat, Pig Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). This antibody is labeled with APC-Cy7.
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A). This antibody is labeled with APC-Cy7.
Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1) Polyclonal Antibody (Human, Rat, Pig), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Rat, Pig Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B (RANk) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II Invariant Chain (MHCDG) Polyclonal Antibody (Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Rat Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Bovine), APC-Cy7
Description: A Rabbit polyclonal antibody against Bovine Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Dog), APC-Cy7
Description: A Rabbit polyclonal antibody against Dog Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Pig), APC-Cy7
Description: A Rabbit polyclonal antibody against Pig Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Rabbit), APC-Cy7
Description: A Guinea polyclonal antibody against Rabbit Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Adaptor Related Protein Complex 2 Mu 1 (AP2m1) Polyclonal Antibody (Human), APC-Cy7
Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Human), APC-Cy7
Description: A Mouse monoclonal antibody against Human Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Rat), APC-Cy7
Description: A Mouse monoclonal antibody against Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) Polyclonal Antibody (Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). This antibody is labeled with APC-Cy7.
Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). This antibody is labeled with APC-Cy7.
Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) Polyclonal Antibody (Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Rat Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). This antibody is labeled with APC-Cy7.
Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A) Polyclonal Antibody (Human, Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Rat Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A). This antibody is labeled with APC-Cy7.
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with APC-Cy7.
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Mouse Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B (RANk) Polyclonal Antibody (Human, Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B (RANk). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Monoclonal Antibody (Rhesus monkey), APC-Cy7
Description: A Mouse monoclonal antibody against Rhesus monkey Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II Invariant Chain (MHCDG) Polyclonal Antibody (Mouse, Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse, Rat Major Histocompatibility Complex Class II Invariant Chain (MHCDG). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Mouse Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Rat ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody (Human, Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Mouse Major Histocompatibility Complex Class II DR Alpha (MHCDRa). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). This antibody is labeled with APC-Cy7.
Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1). This antibody is labeled with APC-Cy7.
Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Monoclonal Antibody (Human, Rat), APC-Cy7
Description: A Mouse monoclonal antibody against Human, Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) Polyclonal Antibody (Human, Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). This antibody is labeled with APC-Cy7.
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Human), APC-Cy7
Description: A Rabbit polyclonal antibody against Human Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with APC-Cy7.
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with APC-Cy7.
Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP) Polyclonal Antibody (Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Mouse Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein (IKBIP). This antibody is labeled with APC-Cy7.
Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) Polyclonal Antibody (Human, Pig), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Pig Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with APC-Cy7.
Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) Polyclonal Antibody (Human, Mouse), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4). This antibody is labeled with APC-Cy7.
Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) Polyclonal Antibody (Human, Rat), APC-Cy7
Description: A Rabbit polyclonal antibody against Human, Rat Actin Related Protein 2/3 Complex Subunit 2 (ARPC2). This antibody is labeled with APC-Cy7.
SEC13 Homolog, Nuclear Pore And COPII Coat Complex Component (SEC13) Antibody
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153)
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat Nuclear pore complex protein Nup153 (NUP153)
Description: Quantitative sandwich ELISA for measuring Rat Nuclear pore complex protein Nup153 (NUP153) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Human Nuclear pore complex protein Nup98- Nup96, NUP98 ELISA KIT
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). This antibody is labeled with APC-Cy7.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). This antibody is labeled with APC-Cy7.
Description: A Mouse monoclonal antibody against Human Serglycin (SRGN). This antibody is labeled with APC-Cy7.
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Konstrukce superskládacího fluorescenčního proteinu řízeného sekrečního expresního systému professional produkci fosfolipázy D u Bacillus subtilis
Fosfolipidy (PL) jsou jednou z hlavních složek v potravinářských a nutraceutických, kosmetických, zemědělských a farmaceutických výrobcích. Fosfolipáza D (PLD) je klíčovým enzymem professional biokatalytickou syntézu nebo modifikaci PL. Zde jsme professional efektivnější přípravu PLD zkonstruovali expresní a sekreční systém PLD v Bacillus subtilis a vyvinuli ekologický reakční systém.
Byla zavedena neklasická sekreční cesta, kde supersložkový zelený fluorescenční protein hraje jako N-koncový vodicí protein. Tento expresní systém může nejen dosáhnout rychlého screeningu expresních kmenů na vysoké úrovni, ale také dosáhnout sekrece cílových proteinů. Za optimálních fermentačních podmínek byla enzymová aktivita kultivačního média 0,35 U / ml, což bylo 2,05násobek aktivity kmenů sekreční dráhy Sec.
Mezitím byly porovnány účinky několika organických rozpouštědel v dvoufázových reakčních médiích. Výsledky ukázaly, že při použití cyklopentylmethyletheru jako organické fáze dosáhla konečná míra konverze 96,9%. Ukázal dobrý aplikační potenciál při syntéze fosfatidylserinu, položil základ professional syntézu a aplikaci dalších vzácných a vysoce hodnotných PL a poskytl odkaz na výrobu dalších biokatalyzátorů.
Exprese proteinu B7-H3 v imunitním kontrolním bodě je spojena se špatným výsledkem a stavem androgenního receptoru u rakoviny prostaty
Pozadí: Nové imunoterapie založené na imunitním kontrolním bodě mohou prospět specifickým skupinám pacientů s rakovinou prostaty, kteří jsou rezistentní na jinou léčbu.
Metody: Imunohistochemicky jsme analyzovali expresi B7-H3, PD-L1 / B7-H1 a androgenového receptoru (AR) ve vzorcích tkáně od 120 pacientů s adenokarcinomem prostaty léčených radikální prostatektomií ve Španělsku a od 206 pacientů s adenokarcinomem prostaty léčených radikální prostatektomie v Norsku.
Výsledky: Exprese B7-H3 pozitivně korelovala s expresí AR a byla spojena s biochemickou recidivou ve španělské kohortě, ale exprese PD-L1 korelovala s žádnou z nich. Nálezy professional B7-H3 byly validovány v norské kohortě, kde exprese B7-H3 pozitivně korelovala se stupněm Gleason, chirurgickými okraji, invazí semenných váčků a rizikovou skupinou CAPRA-S a byla spojena s klinickou recidivou. Vysoká exprese B7-H3 v norské kohortě byla také v souladu s pozitivní expresí AR.
Závěr: Tyto výsledky naznačují zřetelný klinický význam dvou proteinů imunitního kontrolního bodu PD-L1 a B7-H3 u rakoviny prostaty. Naše zjištění zdůrazňují B7-H3 jako použitelný nový protein imunitního kontrolního bodu u rakoviny prostaty.
Blížící se proteinové bariéry: vznikající mechanismy replikace pozastavené u eukaryot
Během replikace nukleární DNA musí multiproteinové replisomové stroje společně procházet a duplikovat celkovou délku každého chromozomu během každého buněčného cyklu. Na určitých místech genomu se replisomy setkávají s těsnými komplexy DNA a proteinů a zpomalují. Tato vidlicová pauza je aktivní proces zahrnující rozpoznání proteinové bariéry blížícím se replisomem prostřednictvím evolučně konzervovaného komplexu vidlice a ochrany (FPC).
Akce FPC chrání vidlice před kolapsem jak u programovaných, tak náhodných proteinových bariér, čímž podporuje integritu genomu. Kromě toho FPC stimuluje kontrolní bod replikace DNA a reguluje topologické přechody poblíž replikační vidlice. Bylo navrženo, aby eukaryotické buňky využívaly fyziologicky naprogramované vidlicové pauzy professional různé účely, jako je udržování počtu kopií na opakujících se lokusech, vyloučení setkání replikace-transkripce, regulace sestavy kinetochore nebo řízení událostí genové konverze během přepínání typu páření.
Zde zkoumáme rostoucí počet přístupů používaných ke studiu pozastavení replikace in vivo a in vitro, jakož i charakterizaci dalších faktorů, které byly nedávno hlášeny okay modulaci pozastavení vidlice v různých systémech. Konkrétně se zaměřujeme na pozitivní roli topoizomeráz při pozastavení vidlí. Popíšeme mannequin, kde postup replisomu je ze své podstaty opatrný, který zajišťuje obecné zachování stability vidlice a integrity genomu, ale může také provádět specializované funkce v určitých lokusech. Dále zdůrazňujeme klasické a nové nevyřešené otázky v této oblasti a navrhujeme místa jejich řešení. Vzhledem okay tomu, jak málo je známo o pozastavení replisomu na proteinových bariérách v lidských buňkách, je zapotřebí více studií zabývajících se tím, jak konzervativní jsou tyto mechanismy.
Funkční průhledy do vysokomolekulární masové vázajícím penicilin proteinů z Streptococcus agalactiae odhalila deleci genu a transposon mutageneze analysisTRANSLATE
Vysokomolekulární proteiny vázající penicilin (PBP) jsou enzymy, které katalyzují biosyntézu peptidoglykanu bakteriální buněčné stěny. Grampozitivní bakteriální patogen Streptococcus agalactiae ( streptokok skupiny B nebo GBS) produkuje pět vysokomolekulárních PBP, jmenovitě PBP1A, PBP1B, PBP2A, PBP2B a PBP2X. Z nich je professional životaschopnost buněk nezbytný pouze PBP2X , zatímco ostatní čtyři PBP jsou jednotlivě postradatelné. Biologická funkce čtyř neesenciálních PBP je v GBS špatně charakterizována. Odstranili jsme pbp1a , pbp1b , pbp2a a pbp2b geny jednotlivě z geneticky dobře charakterizovaného kmene GBS sérotypu V a studoval fenotypy čtyř izogenních mutantních kmenů. Ve srovnání s rodičovským kmenem divokého typu (i) žádný z izogenních mutantních kmenů pbp neměl významný růstový defekt v médiu bohatém na THY, (ii) izogenní mutantní kmeny Δpbp1a a Δpbp1b významně zvýšily citlivost na penicilin a ampicilin a ( iii) izogenní mutantní kmeny Appbp1a a Appbp2b měly významně delší délky řetězce.
Použitím nasycené transpozonové mutageneze a sekvenování místa professional zavedení transpozonu jsme určili geny nezbytné professional životaschopnost kmene GBS divokého typu a každého ze čtyř izogenních kmenů delečních mutantních pbp v médiu bohatém na THY. Pbp1a gen je nezbytný professional životaschopnost buněk v pbp2b vypouští pozadí . Recipročně je pbp2b nezbytný v pozadí odstraňování pbp1a . Gen kódující RodA, peptidoglykan polymerázu, který pracuje ve spojení s PBP2B, je také nezbytný v pozadí delece pbp1a . Naše výsledky společně naznačují funkční překrytí mezi komplexem PBP1A a PBP2B-RodA v biosyntéze peptidoglykanu buněčné stěny GBS.
DŮLEŽITÉ Vysokomolekulární proteiny vázající penicilin (HMM-PBP) jsou enzymy potřebné professional biosyntézu bakteriálních buněčných stěn. Streptokok skupiny B bakteriálních patogenů (GBS) produkuje pět odlišných HMM-PBP. Biologické funkce těchto proteinů nejsou v GBS dobře charakterizovány. V této studii jsme provedli komplexní deleční analýzu genů kódujících HMM-PBP v GBS. Zjistili jsme, že odstranění určitých genů kódujících PBP změnilo bakteriální citlivost na beta-laktamová antibiotika, morfologii buněk a podstatu dalších enzymů zapojených do syntézy peptidoglykanů na buněčné stěně . Výsledky naší studie vrhly nové světlo na biologické funkce PBP v GBS.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins C1/C2(HNRNPC) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2/B1(HNRNPA2B1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
