imobilizace nativních  proteinů  prostřednictvím biokonjugace bez amino katalyzátoru

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imobilizace nativních  proteinů  prostřednictvím biokonjugace bez amino katalyzátoru

Rychle se rozvíjející podskupina SARS-CoV-2 B.1.1.7 ve Spojených státech amerických s hrotovým  proteinem  D178H a  mutacemi membránového  proteinu V70L

Abstrakt Řada SARS-CoV-2 B.1.1.7 je vysoce infekční a od dubna 2021 představovala 92% případů COVID-19 v Evropě a 59% případů COVID-19 v USA. Je definována mutací N501Y v doméně vázající receptor (RBD) proteinu Spike (S) a několika dalších mutacích. Patří mezi ně dvě mutace v N terminální doméně (NTD)S proteinu, HV69-70del a Y144del (také známý jako Y145del kvůli přítomnosti tyrosinu v obou polohách). Nedávno jsme identifikovali několik objevujících se variant SARS-CoV-2, které se vyznačují mutacemi membránových (M) proteinů, včetně I82T a V70L. Nyní identifikujeme podskupinu B.1.1.7, která se objevila prostřednictvím postupných akvizic M: V70L v listopadu 2020, po které následovala nová mutace S: D178H poprvé pozorovaná počátkem února 2021.

Procento izolátů B.1.1.7 v USA, které patří do této podskupiny, se zvýšilo z 0,15% v únoru 2021 na 1,8% v dubnu 2021. Okay dnešnímu dni se tato podskupina jeví jako specifická professional USA s hlášenými případy v 31 státy, včetně Havaje. V dubnu 2021 představoval 36,8% všech izolátů B.1.1.7 ve Washingtonu. Fylogenetická analýza a odvození přenosu s Nextstrain naznačuje, že tato podskupina pravděpodobně pochází buď z Kalifornie, nebo Washingtonu . Strukturální analýza odhalila, že mutace S: D178H je v NTD proteinu S a je blízká dvěma dalším podpisovým mutacím B.1.1.7, HV69-70del a Y144del. Je vystaven povrchu a může změnit NTD terciární konfiguraci nebo přístupnost, a tak má potenciál ovlivnit neutralizaci pomocí NTD namířených protilátek.

 

Rychlé trojrozměrné stanovení tvaru globulárních  proteinů  mobilitou kapilární elektroforézou a nativní hmotnostní spektrometrií

Zavedené vysoce výkonné proteomické metody poskytují omezené informace o stereostrukturách proteinů. Tradiční technologie professional stanovení proteinové struktury obvykle vyžadují pracné kroky a nelze je provádět vysoce výkonným způsobem. Zde uvádíme novou metodu středního výkonu spojením mobility kapilární elektroforézy (MCE) a nativní hmotnostní spektrometrie (MS) professional trojrozměrné (3D) stanovení tvaru globulárních proteinů v kapalné fázi , která poskytuje jak geometrickou strukturu, tak molekulární hromadné informace o bílkovinách.

Byla vytvořena teorie ke korelaci iontového hydrodynamického poloměru a distribuce stavu náboje v nativním hmotnostním spektru s geometrickými parametry proteinu, pomocí kterých lze určit strukturu (tvar) proteinu s nízkým rozlišením. Naše testovací údaje z 11 různých globulárních proteinů ukázaly, že tento přístup nám umožňuje určit tvary jednotlivých proteinů, proteinových komplexů a proteinů ve směsi a sledovat konformační změny proteinů. Kromě poskytování doplňkových informací o struktuře proteinů a schopnosti analýzy směsi je tato metoda založená na MCE a nativní MS rychlá a má nízkou spotřebu vzorků, takže je potenciálně použitelná v proteomice a strukturní biologii shora dolů professional intaktní analýzu globulárního proteinu nebo komplexu proteinů.

 

Rychlá povrchová imobilizace nativních  proteinů  prostřednictvím biokonjugace bez amino katalyzátoru bez katalyzátoru

Imobilizace na povrchu poskytuje užitečnou platformu professional biosenzování, screening léků, tkáňové inženýrství a další chemické a biologické aplikace. Nicméně , některé z použitých reakcí jsou neefektivní a / nebo složité, což omezuje jejich aplikace v imobilizaci. Zde používáme spontánní a bezkatalyzátorovou biokonjugaci amino-ynového kliknutí ke generování aktivovaných povrchů funkčních skupin ethynylové skupiny professional rychlou imobilizaci nativních proteinů a buněk.

Biomolekuly, jako je hovězí sérový albumin (BSA) , lidský IgG a peptid C (RGDfK), mohly být kovalentně imobilizovány na povrchech za pouhých 30 minut. Je pozoruhodné, že bioaktivita ukotvených biomolekul zůstává nedotčena, což se ověřuje účinným zachycením cílových protilátek a buněk z objemových roztoků. Tato strategie představuje alternativu okay vysoce účinné povrchové biofunkcionalizaci.

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Description: Antibody raised against GPR101-Specific

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Description: Antibody raised against HBM-Specific

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Description: Antibody raised against HBQ1-Specific

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Description: Antibody raised against HBZ-Specific

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Description: Antibody raised against HDAC1-specific

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Description: Antibody raised against HDAC2-specific

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Description: Antibody raised against HDAC3-specific

Na vazebné reakce loratadinu s lidským sérem akutní fáze  proteinu  glyko alfa 1-kyselý protein

Loratadin je důležité antialergické léčivo. Je to antihistaminikum druhé generace používané okay léčbě alergické rýmy, senné rýmy a kopřivky. Lidské sérum alfa 1-kyselý glykoprotein (AG) je důležitým proteinem akutní fáze a bylo zjištěno, že jeho sérová koncentrace stoupá při zánětu a akutní reakci. Vazebná interakce mezi loratadinem a AG je studována pomocí spektroskopie a technik molekulárního dokování. Výsledky získané z experimentů zhášení fluorescence prokázaly, že intenzita fluorescence AG je zhášena loratadinem. Loratadin bylo zjištěno, že váže AG s vazebnou konstantou ≈10 4  při 298 Okay.

Bylo zjištěno, že Gibbova volná energie je negativní professional interakci loratadinu s AG, což naznačuje, že proces vazby je spontánní. Vazba loratadinu s AG vyvolala uspořádané struktury v proteinu. Vodíková vazba a hydrofobní interakce byly hlavními vazebnými silami mezi AG-loratadinem, jak vyplývá z výsledků molekulárního dokování. Tato studie naznačuje důležitost prostorové vazby antialergického léčiva na AG u onemocnění, kde plazmatická koncentrace AG mnohonásobně stoupá a interakce s tímto proteinem se stává významnou. Tato studie pomůže při navrhování dávkování léku a odpovídající úpravě okay dosažení optimálního výsledku léčby. Sdělil Ramaswamy H. Sarma.

Cílená   degradace proteinů prostřednictvím rychlé optogenetické aktivace a její aplikace na kontrolu buněčné signalizace

Vývoj metodik professional opticky spouštěnou degradaci proteinů umožňuje studium dynamických proteinových funkcí, jako jsou ty, které se účastní buněčné signalizace , které je obtížné zkoumat tradičními genetickými technikami. Zde popisujeme design a implementaci nového světelně řízeného peptidového degronu, který uděluje degradaci dráhy N-konce na jeho proteinový cíl. Degron zahrnuje fotocaged N-koncovou aminokyselinu a linker bohatý na lysin, 13 zbytků. Umístěním do N-koncového zbytku jsme byli schopni opticky řídit rozpoznávání N-degronu ligázou E3, což následně řídilo ubikvitinaci a proteazomální degradaci cílového proteinu.

Prokazujeme širokou použitelnost aplikací tohoto přístupu na různorodou sadu cílových proteinů, včetně EGFP, luciferázy světlušek, kinázy MEK1 a fosfatázy DUSP6 (také známé jako MKP3). Degron v kleci lze použít s minimálním proteinovým inženýrstvím a poskytuje prakticky úplnou, světlem spouštěnou degradaci proteinu v sekundovém časovém měřítku.

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Glutathione S-Transferase (GST) Antibody

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Glutathione S-Transferase

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Mouse alpha-glutathione S-transferase (alpha-GST) ELISA Kit

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Rat alpha-glutathione S-transferase (alpha-GST) ELISA Kit

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Human alpha-glutathione S-transferase (alpha-GST) ELISA Kit

abx257305-96tests 96 tests
EUR 676

Schistosoma japonicum Glutathione S-Transferase(GST) Antibody

32839-05111 150 ug
EUR 261

Glutathione-S-transferase (GST) Peroxidase Conjugated Antibody

20-abx137176
  • EUR 551.00
  • EUR 871.00
  • EUR 1720.00
  • 100 ul
  • 200 ul
  • 500 ul

Glutathione-S-transferase (GST) Affinity Purified Antibody

20-abx137511
  • EUR 829.00
  • EUR 328.00
  • EUR 217.00
  • 100 ug
  • 20 ug
  • 5 ug

Glutathione S-Transferase Pi (GST pi) Antibody

abx020559-10ug 10 ug
EUR 342

Recombinant (E.Coli, GST tag) Glutathione S-Transferase

RP-780 5 ug
EUR 164

Glutathione S-Transferase Fluorescent Kit

K008-F1 1x96 well plate
EUR 349

Glutathione S-Transferase Activity Kit

SKT-203-96 1 plate of 96 wells
EUR 428
Description: Enzyme Activity Fluorometric assay used for quantitative measuring the activity of glutathione S-transferase in Urine, Serum, EDTA Plasma, Heparin Plasma, Cell Lysates samples from Human, Mollusk, Fish

Glutathione S-transferase Assay Kit

abx298813-100Assays 100 Assays
EUR 410

Glutathione S-Transferase (Recombinant)

20-abx073059
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

Glutathione-S-transferase Antibody

20-abx137109
  • EUR 523.00
  • EUR 940.00
  • EUR 356.00
  • 0.2 mg
  • 0.5 mg
  • 100 ug

Recombinant Glutathione S-Transferase

7-02872 10µg Ask for price

Recombinant Glutathione S-Transferase

7-02873 50µg Ask for price

Recombinant Glutathione S-Transferase

7-02874 1mg Ask for price

Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum

GSTA11-S 100 ul
EUR 457

Rabbit Anti-Human Glutathione Transferase alpha (GST-alpha) antiserum

GSTA12-S 100 ul
EUR 457

Rabbit Anti-Rat Glutathione Transferase mu (GST-mu) antiserum

GSTM11-S 100 ul
EUR 457

Rabbit Anti-Human Glutathione Transferase mu (GST-mu) antiserum

GSTM12-S 100 ul
EUR 457

Rabbit Anti-Human Glutathione Transferase Pi (GST-pi) antiserum

GSTP12-S 100 ul
EUR 457

GST, Glutathione S-Transferase Recombinant Protein, His Tag

PROTP26624-1 Regular: 50ug
EUR 317
Description: Recombinant Schistosoma japonicum GST full length protein contains 244 amino acids(1-224 a.a.) expressed in E.coli, having a molecular mass of 28.3kDa. ;The GST protein is fused to a 20 amino acids His-Tag at N-terminus. ;The GST protein is purified by proprietary chromatographic techniques.

ELISA kit for Human GST?1 (Glutathione S Transferase Alpha 1)

E-EL-H1333 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?1 (Glutathione S Transferase Alpha 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human GST?1 (Glutathione S Transferase Omega 1)

E-EL-H1336 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?1 (Glutathione S Transferase Omega 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human GST?5 (Glutathione S Transferase Alpha 5)

E-EL-H1346 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?5 (Glutathione S Transferase Alpha 5) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human GST?1 (Glutathione S Transferase Kappa 1)

E-EL-H1402 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?1 (Glutathione S Transferase Kappa 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human GST?4 (Glutathione S Transferase Alpha 4)

E-EL-H1404 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?4 (Glutathione S Transferase Alpha 4) in samples from Serum, Plasma, Cell supernatant

QuantiChrom Glutathione S-transferase Assay Kit

DGST-100 100
EUR 318
Description: Glutathione S-transferase activity determination in biological samples. Method: OD340nm; Samples: Biological samples (e.g. cell lysates, tissues, etc.); Species: All; Procedure: 10 min; Size: 100 tests; Detection Limit: 2 U/L; Shelf Life: 6 months

Blattella germanica Glutathione S-transferase

1-CSB-YP522732BTL
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Blattella germanica Glutathione S-transferase expressed in Yeast

Glutathione S-Transferase Standard, 50UL

C026-50UL 50UL
EUR 123

Blattella germanica Glutathione S-transferase

1-CSB-EP522732BTL
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Blattella germanica Glutathione S-transferase expressed in E.coli

Glutathione S-Transferase M1 (Recombinant)

20-abx073076
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

Glutathione S-Transferase M1 (Recombinant)

20-abx073901
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg

anti-Glutathione S Transferase alpha

YF-PA12145 50 ul
EUR 363
Description: Mouse polyclonal to Glutathione S Transferase alpha

anti-Glutathione S Transferase alpha

YF-PA12146 50 ug
EUR 363
Description: Mouse polyclonal to Glutathione S Transferase alpha

anti-Glutathione S Transferase alpha

YF-PA12147 100 ul
EUR 403
Description: Rabbit polyclonal to Glutathione S Transferase alpha

anti-Glutathione S Transferase alpha

YF-PA12148 100 ug
EUR 403
Description: Rabbit polyclonal to Glutathione S Transferase alpha

Glutathione S Transferase (GST) Tag Monoclonal Antibody (Pan-species)

4-TAX158Ge22
  • EUR 234.00
  • EUR 2312.00
  • EUR 580.00
  • EUR 292.00
  • EUR 208.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Pan-species Glutathione S Transferase (GST) Tag

Schistosoma japonicum Glutathione S-Transferase(GST) Antibody (Biotin Conjugate)

32839-05121 150 ug
EUR 369

Goat Anti-Glutathione Transferase (GST, S. japonicum) IgG# 3

GST13-A 1 mg
EUR 408

Glutathione Transferase (GST, S. japonicum, ) Protein-Agarose (Aff matrix)

GST15R-AS 0.50 ml
EUR 347

ELISA kit for Human GST?1/GSTt1 (Glutathione S Transferase Theta 1)

E-EL-H1334 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?1/GSTt1 (Glutathione S Transferase Theta 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human GST?2/GSTt2 (Glutathione S Transferase Theta 2)

E-EL-H1335 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Human GST?2/GSTt2 (Glutathione S Transferase Theta 2) in samples from Serum, Plasma, Cell supernatant

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Hu-10x96wellstestplate 10x96-wells test plate
EUR 4502.43
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glutathione S Transferase Pi (GSTp) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Hu-1x48wellstestplate 1x48-wells test plate
EUR 458.44
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glutathione S Transferase Pi (GSTp) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Hu-1x96wellstestplate 1x96-wells test plate
EUR 612.05
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glutathione S Transferase Pi (GSTp) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Hu-5x96wellstestplate 5x96-wells test plate
EUR 2454.23
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glutathione S Transferase Pi (GSTp) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

4-SEB090Hu
  • EUR 4553.00
  • EUR 2405.00
  • EUR 613.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Glutathione S Transferase Pi (GSTp) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Pig Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Po-10x96wellstestplate 10x96-wells test plate
EUR 5372.91
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Glutathione S Transferase Pi (GSTp) in serum, plasma and other biological fluids.

Pig Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Po-1x48wellstestplate 1x48-wells test plate
EUR 530.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Glutathione S Transferase Pi (GSTp) in serum, plasma and other biological fluids.

Pig Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Po-1x96wellstestplate 1x96-wells test plate
EUR 714.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Glutathione S Transferase Pi (GSTp) in serum, plasma and other biological fluids.

Pig Glutathione S Transferase Pi (GSTp) ELISA Kit

SEB090Po-5x96wellstestplate 5x96-wells test plate
EUR 2915.07
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Glutathione S Transferase Pi (GSTp) in serum, plasma and other biological fluids.

Pig Glutathione S Transferase Pi (GSTp) ELISA Kit

4-SEB090Po
  • EUR 5423.00
  • EUR 2866.00
  • EUR 715.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Glutathione S Transferase Pi (GSTp) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Mouse Glutathione S-transferase P(GSTP1) ELISA kit

CSB-EL009989MO-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Mouse Glutathione S-transferase P (GSTP1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Glutathione S-transferase P(GSTP1) ELISA kit

1-CSB-EL009989MO
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse Glutathione S-transferase P(GSTP1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Glutathione S-transferase P(GSTP1) ELISA kit

CSB-EL009989RA-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Rat Glutathione S-transferase P (GSTP1) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Glutathione S-transferase P(GSTP1) ELISA kit

1-CSB-EL009989RA
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Glutathione S-transferase P(GSTP1) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

RD-GSTp-Hu-48Tests 48 Tests
EUR 500

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

RD-GSTp-Hu-96Tests 96 Tests
EUR 692

Porcine Glutathione S Transferase Pi (GSTp) ELISA Kit

RD-GSTp-p-48Tests 48 Tests
EUR 580

Porcine Glutathione S Transferase Pi (GSTp) ELISA Kit

RD-GSTp-p-96Tests 96 Tests
EUR 807

Pig GSTP1/ Glutathione S-transferase P ELISA Kit

E0082Pi 1 Kit
EUR 717

Mouse Gsta2/ Glutathione S-transferase A2 ELISA Kit

E0636Mo 1 Kit
EUR 632

Human GSTA/ Glutathione S-transferase A ELISA Kit

E1069Hu 1 Kit
EUR 605

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